Title:
ISOLATED INTERNAL NUCLEAR MATRIX PROTEIN AND UTILIZATION THEREOF
Document Type and Number:
Japanese Patent JP2675774
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a protein useful for characterizing the degree of malignancy of a tissue derived from a cell and/or the cell and to provide a method for utilization thereof.
SOLUTION: This internal nuclear matrix protein is isolated so as not to contain an external nuclear matrix protein. In this case, the internal nuclear matrix protein is a protein capable of maintaining the solubility when dialyzing an 8 M urea solution of a nuclear matrix protein without containing a protein of a cell skeleton and a chromatinic protein against a physiological buffer. The external nuclear matrix protein is a protein becoming insoluble under similar conditions. A method for detecting a compound binding to the isolated internal nuclear matrix protein comprises (a) a step for reacting the isolated internal nuclear matrix protein with a selected compound and (b) a step for detecting the presence of the binding of the protein to the compound.
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Inventors:
Edward G. Huey
Larry Edwards
Larry Edwards
Application Number:
JP2289596A
Publication Date:
November 12, 1997
Filing Date:
February 08, 1996
Export Citation:
Assignee:
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
International Classes:
C07K1/12; C07K1/14; C07K1/36; C07K14/435; C12P21/00; C12Q1/00; G01N33/50; C12Q1/04; C12Q1/68; G01N33/53; G01N33/68; (IPC1-7): C07K14/435; C07K1/12; C07K1/14; C07K1/36; C12P21/00; C12Q1/04
Attorney, Agent or Firm:
Shusaku Yamamoto