Title:
分泌タンパク質を発現する細胞の単離
Document Type and Number:
Japanese Patent JP4323167
Kind Code:
B2
Abstract:
A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.
Inventors:
Foundle, James P.
Neil, Star
Chain, gun
Jankopoulos, George Dee.
Neil, Star
Chain, gun
Jankopoulos, George Dee.
Application Number:
JP2002558477A
Publication Date:
September 02, 2009
Filing Date:
January 16, 2002
Export Citation:
Assignee:
REGENERON PHARMACEUTICALS, INC.
International Classes:
A01K67/027; C12N15/09; C07K14/315; C07K14/52; C07K16/28; C12N5/10; C12Q1/02; C40B30/04; G01N33/569; G01N33/68
Domestic Patent References:
| JP8504574A |
Foreign References:
| WO1999058977A1 | ||||
| WO1998023964A1 |
Other References:
Proc Natl Acad Sci U S A.,1995年,vol.92, no.6,p.1921-1925
Attorney, Agent or Firm:
Takeshi Oshio