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Title:
ISOLATION OF CROSSBREED CELL
Document Type and Number:
Japanese Patent JPS6041480
Kind Code:
A
Abstract:
A method for isolating living, isolatable hybrid cells of a continuous dividing cell (e.g. a malignant myeloma cell) and a normal cell (incapable of continuous division) wherein hybridization is conducted in discrete, individual reaction chambers containing a predetermined number of continuous dividing cells and normal cells such that only a single hybrid therebetween can form in any chamber. The method insures that, after suitable isolation procedures, each identified living hybridoma necessarily will have been derived from a single hybrid cell of a continuous dividing cell and a normal cell and, thus, is capable of producing only a single, monoclonal product.

Inventors:
SAMU ROOZU
Application Number:
JP6309484A
Publication Date:
March 05, 1985
Filing Date:
March 30, 1984
Export Citation:
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Assignee:
BIO RESPONSE INC
International Classes:
C12N15/02; A61K39/395; C12N5/10; C12N5/16; C12N11/04; C12P21/08; C12Q1/04; C12Q1/24; G01N33/574; G01N33/577; C12R1/91; (IPC1-7): A61K39/395; C12N5/00; C12N15/00; C12Q1/04; C12R1/91; G01N33/574
Attorney, Agent or Firm:
Takehiko Suzue