Title:
ケトレダクターゼ変異体及びその応用
Document Type and Number:
Japanese Patent JP6988000
Kind Code:
B2
Abstract:
A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence. The ketoreductase mutants have high stability, especially the resistance to acetone and isopropanol such that when the mutants are used in the preparation of a chiral hydroxyl heterocyclic substance, the components of the reaction system are simplified, costs are lowered, and the prepared chiral alcohol has an ee value up to 99.8%.
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Inventors:
Hong, Hao
James, Gage
Lou, Jiang Ping
Shoe, Shinfu
You, Wenyang
Jean, Na
Ma, Ouray
Chen, Ebin
Mu, Feuillant
James, Gage
Lou, Jiang Ping
Shoe, Shinfu
You, Wenyang
Jean, Na
Ma, Ouray
Chen, Ebin
Mu, Feuillant
Application Number:
JP2020537527A
Publication Date:
January 05, 2022
Filing Date:
January 22, 2018
Export Citation:
Assignee:
JILIN ASYMCHEM LABORATORIES CO., LTD.
International Classes:
C12N9/04; C12N1/19; C12N1/21; C12N15/53; C12P17/00; C12P17/04
Domestic Patent References:
| JP2010536385A |
Foreign References:
| CN107586793A |
Attorney, Agent or Firm:
Hiraki International Patent Office