Title:
KILLER YEAST UNASSIMILABLE TO LACTIC ACID
Document Type and Number:
Japanese Patent JP3870248
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To prevent occurrence of lactic acid metabolism causing aerobic deterioration and prevent aerobic deterioration of silage by adding the subject yeast to silage.
SOLUTION: This killer yeast which is unassimilable to lactic acid loses phosphoenolpyruvic acid carboxynase (PEPCK) gene. The killer yeast is added to silage. The killer yeast is obtained by method, e.g. introducing a plasmid pKP2, etc., in which BgI II fragment of PEPCK gene is lost and a maker gene is inserted in stead of the gene into the killer yeast K. lactis IFO 1267 strain, etc. The killer yeast block a gluconeogenesis system and is not grown by using lactic acid as a carbon source.
Inventors:
Hiroko Kitamoto
Otomo Sadayo
Otomo Sadayo
Application Number:
JP20509296A
Publication Date:
January 17, 2007
Filing Date:
August 02, 1996
Export Citation:
Assignee:
Centro Internacional de Agricultura Tropical
International Classes:
C12N1/19; C07H21/04; C12N15/09; C12R1/645; (IPC1-7): C12N1/19; A23K3/02; C07H21/04; C12N15/09; //(C12N1/19; C12R1:645)
Other References:
J. Dairy Sci., 1993,Vol.76,No.3,p.803-811
Folia Microbiologica,1994,Vol.39,No.6,p.512
Nucleic Acids Res.,1988,Vol.16,No.22,p.10926
日本農芸化学会大会講演要旨集,1994,p.116,2Ua5
Folia Microbiologica,1994,Vol.39,No.6,p.512
Nucleic Acids Res.,1988,Vol.16,No.22,p.10926
日本農芸化学会大会講演要旨集,1994,p.116,2Ua5
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita
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