To obtain a new kojibiose phosphorylase having action of decomposing kojibiose in the presence of an inorganic phosphoric acid (salt) and producing D-glucose, etc., and useful for foods, cosmetics and pharmaceutical fields, etc.
This kojibiose phosphorylase has action of decomposing kojibiose in the presence of an inorganic phosphoric acid (salt) and producing D-glucose and β-D-glucose-1 phosphoric acid (salt) and has the following physicochemical properties: Molecular weight, 83,000 ± 5,000 Dalton (by SDS gel electrophoresis); optimum temperature, nearly 65°C when reacted at pH 5. 5 for 30 min; optimum pH, nearly 5.5 when reacted at 60°C for 30 min; temperature stability, stable up to nearly 65°C when retained at pH 5. 5 for 1 hr; pH stability, pH 5.5-10.0 when retained at 4°C for 24 hr. Furthermore, the kojibiose phosphorylase contains one or plural amino acid sequences among amino acid sequence, etc., represented by formula I as a partial amino acid sequence and has an amino acid sequence of formula II and has kojibiose phosphorylase-producing ability. The kojibiose phosphorylase is obtained by culturing a microorganism such as Thermoanaerobium brockii.
KUBOTA MICHIO
CHAEN HIROTO
MIYAKE TOSHIO
