LYTIC PROTEIN DERIVED FROM BACTERIOPHAGE PARASITIC TO PALSTONIA SOLANACEARUM, DNA CODING FOR THE PROTEIN, AND TRANSFORMANT HAVING THE DNA

Title:

LYTIC PROTEIN DERIVED FROM BACTERIOPHAGE PARASITIC TO PALSTONIA SOLANACEARUM, DNA CODING FOR THE PROTEIN, AND TRANSFORMANT HAVING THE DNA

Document Type and Number:

Japanese Patent JP3935279

Kind Code:

B2

Abstract:

PROBLEM TO BE SOLVED: To obtain a new lytic protein which is produced by a bacteriophage parasitic to Ralstonia solanacearum, which has a specific molecular weight and an N-terminal amino acid sequence, and can be used for preparing a transformed plant useful in agriculture and so on.
SOLUTION: A new lytic protein is provided which is produced by a bacteriophage parasitic to Ralstonia solanacearum, has a molecular weight of about 71 kDa as determined by SDS-PAGE and an N-terminal amino acid sequence shown in the formula, and can give resistance against the bacterial wilt of eggplants and to bacco plants. A gene coding for the protein is useful for preparing a transformed plant useful in agriculture and so on. This lytic protein is obtained by applying the ammonium sulfate precipitation to a suspension of Ralstonia solanacearum having bacteriophage to recover a 0-40% saturated ammonium sulfate fraction, followed by applying the obtained fraction to the gel filtration high-performance liquid chromatography.

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Inventors:

Ozawa Hidenori
Hiroshi Tanaka
Tetsuji Yamada

Application Number:

JP32586698A

Publication Date:

June 20, 2007

Filing Date:

October 30, 1998

Export Citation:

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Assignee:

Japan Tobacco Inc.

International Classes:

C12N15/09; A01H5/00; C12N5/10; C12N9/36; C12R1/91; (IPC1-7): C12N15/09; A01H5/00; C12N5/10; C12N9/36; //(C12N9/36; C12R1:91)

Domestic Patent References:

JP4048762B2
JP6017291B2

Other References:

Mol. Genet. Genomics, vol.265, p.95-101, (2001)
Ann. Phytopath. Soc. Japan, vol.56, p.243-246, (1990)

Attorney, Agent or Firm:

Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Tadahiko Kurita
Yasushi Kobayashi
Makoto Aihara

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