To provide a method for producing a purified β-D-glucan, with which a β-D-glucan is separated from a microorganism culture solution containing a β-D-glucan and purified, and which is a method for obtaining a β-D-glucan having high transparency and to obtain a purified microorganism-derived β-D-glucan having high transparency.
The method for producing a purified β-D-glucan comprises a first process for adjusting a microorganism culture solution containing a β-D-glucan or a ground microorganism solution, a second process for removing a microorganism or an insoluble admixture to give a supernatant and a third process for ultrafiltering the supernatant in alkalinity so as to remove the whole or a part of a low-molecular admixture from the β-D-glucan. The purified β-D-glucan having light transmittance of ≥60% at 660 nm in 0.2 wt.% of an aqueous solution at 25°C is obtained by the method.
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SUZUKI TOSHIO
SUZUKI TAKAHIRO
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Kakehi Yuro
Yoko Tada