Title:
METHODS FOR PURIFYING AND QUANTITATIVELY DETERMINING Fc-BINDING PROTEIN
Document Type and Number:
Japanese Patent JP2014125437
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for purifying Fc-binding protein to high purity by using cation exchange chromatography and also to provide a simple and accurate method for quantitatively determining the amount of Fc-binding protein.SOLUTION: The problem is solved by adding 0.12 mol/L - 0.18 mol/L of arginine at least to a sample solution containing Fc-binding protein for purification by cation exchange chromatography, or to a sample solution for quantitative determination of Fc-binding protein.
Inventors:
HANZAWA SATOSHI
TERAO YOSUKE
TERAO YOSUKE
Application Number:
JP2012281169A
Publication Date:
July 07, 2014
Filing Date:
December 25, 2012
Export Citation:
Assignee:
TOSOH CORP
International Classes:
C07K1/18; C07K14/705; G01N30/00; G01N30/02; G01N30/26; G01N30/50; G01N30/86; G01N30/88; C12N15/09
Domestic Patent References:
| JP2010516769A | 2010-05-20 | |||
| JP2012250945A | 2012-12-20 | |||
| JP2003510368A | 2003-03-18 | |||
| JP2011206046A | 2011-10-20 |
Foreign References:
| WO2012078376A1 | 2012-06-14 | |||
| WO2010071208A1 | 2010-06-24 |
Other References:
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荒川力, 薬学雑誌, vol. 130, no. 6, JPN6017013203, 2010, pages 793 - 800, ISSN: 0003569256
ARAKAWA, T. ET AL., J. PHARM. SCI., vol. 99, no. 4, JPN6017013204, 2010, pages 1674 - 1692, ISSN: 0003569257
EJIMA, D. ET AL., J. CHROMATOGR. A, vol. 1094, JPN6017013205, 2005, pages 49 - 55, ISSN: 0003569258
YUMIOKA, R. ET AL., PROTEIN EXPR. PURIF., vol. 70, JPN6017013206, 2010, pages 218 - 223, ISSN: 0003569259
ARAKAWA, T. ET AL., PROTEIN EXPR. PURIF., vol. 54, JPN6016035348, 2007, pages 110 - 116, ISSN: 0003569260