Title:
PRODUCTION OF CARRIER-PEPTIDE CONJUGATES USING CHEMICALLY REACTIVE UNNATURAL AMINO ACIDS
Document Type and Number:
Japanese Patent JP2016155835
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide efficient, cost-effective, and large-scale methods of manufacturing carrier polypeptide.SOLUTION: The invention relates to methods comprising incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, reacting the first and second unnatural amino acids to produce the conjugate, and further making a covalently coupled carrier polypeptide-target polypeptide conjugate. The invention also relates to orthogonal translation systems in methylotrophic yeast and to methods of producing carrier and target polypeptide variants containing unnatural amino acids using these systems.SELECTED DRAWING: None
Inventors:
TRAVIS YOUNG
PETER G SCHULTZ
PETER G SCHULTZ
Application Number:
JP2016053368A
Publication Date:
September 01, 2016
Filing Date:
March 17, 2016
Export Citation:
Assignee:
SCRIPPS RESEARCH INST
International Classes:
C07K19/00; A61K38/00; A61K47/42; A61K47/48; C12N1/19; C12N15/09
Domestic Patent References:
| JP2007525943A | 2007-09-13 | |||
| JP2007536899A | 2007-12-20 | |||
| JP2008525024A | 2008-07-17 |
Foreign References:
| WO2007130453A2 | 2007-11-15 | |||
| WO2008077079A1 | 2008-06-26 |
Other References:
日本化学会講演予稿集, vol. 83, no. 2, JPN6017004482, 2003, pages 1140, ISSN: 0003673295
Attorney, Agent or Firm:
Junji Yuda
Noriyuki Takebayashi
Noriyuki Takebayashi
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