Title:
毛様体周縁部様構造体の製造法
Document Type and Number:
Japanese Patent JP6991517
Kind Code:
B2
Abstract:
The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more and 100% or less of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway and a substance inhibiting the FGF signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the "cell aggregate in which a RPE65 gene-expressing cell does not appear" thus obtained in a serum-free medium or serum-containing medium each free of a substance acting on the Wnt signal pathway and so on. According to the production method of the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.
Inventors:
Atsushi Kuwahara
Yoshiki Sasai
Yoshiki Sasai
Application Number:
JP2019042043A
Publication Date:
February 15, 2022
Filing Date:
March 07, 2019
Export Citation:
Assignee:
Sumitomo Chemical Co., Ltd.
RIKEN
RIKEN
International Classes:
C12N5/071; C12N5/0735
Domestic Patent References:
| JP2013128474A | ||||
| JP2013128477A | ||||
| JP2013128476A | ||||
| JP2013128475A | ||||
| JP2013201943A |
Foreign References:
| WO2013077425A1 |
Other References:
T.Nakano, et al,Cell Stem Cell,2012年,Vol.10,p.771-785
T.Inoue, et al,STEM CELLS,2006年,Vol.24,p.95-104
S.Fuhrmann,Organogenesis,2008年,Vol.4, Issue 2,p.60-67
T.Inoue, et al,STEM CELLS,2006年,Vol.24,p.95-104
S.Fuhrmann,Organogenesis,2008年,Vol.4, Issue 2,p.60-67
Attorney, Agent or Firm:
Takashima Hajime
Mitsuyoshi Kamada
Mitsuyoshi Kamada