Title:
MANUFACTURING METHOD OF USEFUL PROTEIN
Document Type and Number:
Japanese Patent JP2007176831
Kind Code:
A
Abstract:
To develop a simple recovering method of a recombinant protein contained in silkworm silk yarn thereby to solve problems for putting it into practical use in industrial applications.
The recombinant protein produced in silk yarn by a transgenic silkworm is recovered at high purity without a complicated process for protein purification by dissolving the silk yarn in a solution of a neutral salt, an alkaline solution of copper or a urea solution and subsequently dialyzing or diluting with an aqueous solution of a low salt concentration thereby causing the silk yarn protein to precipitate.
Inventors:
MORITA TAKESHI
TANAKA TAKASHI
TANAKA TAKASHI
Application Number:
JP2005375161A
Publication Date:
July 12, 2007
Filing Date:
December 27, 2005
Export Citation:
Assignee:
TORAY INDUSTRIES
International Classes:
C07K14/435; C07K1/14; C07K14/015; C07K14/52; C07K14/555; C12N15/09
Previous Patent: JP2007176830
Next Patent: SKIN AGEING-PREVENTING COMPOSITION CONTAINING EXTRACT OF APHANIZOMENON
Next Patent: SKIN AGEING-PREVENTING COMPOSITION CONTAINING EXTRACT OF APHANIZOMENON