MEASUREMENT, OF HGV GENE BY REAL TIME DETECTION PCR METHOD, AND PRIMER AND PROBE TO BE USED THEREFOR

Japanese Patent JPH10248580

A

To obtain a new primer for highly sensitive and accurate detection of hepatitis G virus(HGV), etc., useful for measurement of HGV gene by a real time detection PCR method, etc., consisting of an oligonucleotide having a specific base sequence.

The primer is a new forward-side primer as, an oligonucleotide having the base sequence consisting of consecutive 15-39 bases of the base sequence expressed by formula I, to be used for measurement of hepatitis G virus(HGV) gene by a real time detection PCR method and a new reverse-side primer as, a second oligonucleotide having the base sequence consisting of consecutive 15-39 bases of the base sequence expressed by formula II to be used for measurement of HGV gene by a real time detection PCR method, and can measure HGV in a highly sensitive, accurate and easy way. This primer is obtained by chemically synthesizing forward-side and reverse-side DNAs of the base sequence of HGV genome.

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OBARA MICHINORI
INOUE KAZUAKI
KATSUME ASAO
TAKEUCHI TOMOKO
KAWAGUCHI RYUJI

JP6733197A

September 22, 1998

March 05, 1997

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TOKYO MET GOV RINSHIYOU IGAKU
S R L KK

G01N21/78; C07H21/02; C07H21/04; C12N15/09; C12Q1/68; (IPC1-7): C12N15/09; C07H21/02; C07H21/04; C12Q1/68; G01N21/78

Eijiro Tanigawa

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