Title:
タンパク質が結合した作用物質化合物のインタクト質量の測定
Document Type and Number:
Japanese Patent JP6997271
Kind Code:
B2
Abstract:
The present invention provides methods and systems for the rapid determination of the intact mass of non-covalently associated antibody heavy chains (HC) and light chains (LC) which result from the attachment of drug conjugates to interchain cysteine residues. By analyzing the antibody-drug conjugate (ADC) using native desalting conditions, the intact-bivalent structure of the ADC, which ordinarily would decompose as a consequence of denaturing chromatographic conditions typically used for LCMS, is maintained. The mass of the desalted ADC is subsequently determined using desolvation and ionization ESI-MS conditions. The methods described herein provide for direct measurement of the intact mass of an ADC conjugated at interchain cysteine residues. The methods described herein also provide for the relative quantitation of the individual ADC species.
Inventors:
John Fay Barriere-Douglas
Oscar Saras
Oscar Saras
Application Number:
JP2020159739A
Publication Date:
January 17, 2022
Filing Date:
September 24, 2020
Export Citation:
Assignee:
Siegen Incorporated
International Classes:
G01N27/62; G01N30/72; G01N30/88
Domestic Patent References:
| JP2007532882A | ||||
| JP2005524086A | ||||
| JP2002214195A | ||||
| JP10509028A |
Foreign References:
| US20090325331 |
Attorney, Agent or Firm:
Aoki Atsushi
Fukumoto product
Tetsuji Koga
Youichi Watanabe
Ryotaro Takei
Hidenao Tsuda
Fukumoto product
Tetsuji Koga
Youichi Watanabe
Ryotaro Takei
Hidenao Tsuda