Title:
酵素を用いた測定方法
Document Type and Number:
Japanese Patent JP5858945
Kind Code:
B2
Abstract:
A method for measuring an analyte is described that includes the steps of i) preparing a reagent (D) in which an enzyme (A) and an enzyme (B) coexist in the absence of the analyte; ii) bringing the analyte into contact with the enzyme (A) and the enzyme (B) so that the enzyme (A) acts on the analyte to produce a product (E), on which the enzyme (B) does not substantially act, from the analyte; iii) producing a product (C) by allowing the enzyme (A) or an enzyme (F) that is different from the enzyme (A) to act on the analyte and/or the product (E); and iv) detecting the product (C) by the enzyme (B).
Inventors:
Sugiyama Koji
Yonehara Satoshi
Kazuhiko Shimoji
Yonehara Satoshi
Kazuhiko Shimoji
Application Number:
JP2013045758A
Publication Date:
February 10, 2016
Filing Date:
March 07, 2013
Export Citation:
Assignee:
Arkray Co., Ltd.
Kikkoman Biochemifa Co., Ltd.
Kikkoman Biochemifa Co., Ltd.
International Classes:
C12Q1/26; C12Q1/37; G01N33/48; G01N33/68
Domestic Patent References:
| JP2008245657A | ||||
| JP2004004071A | ||||
| JP2000253898A |
Foreign References:
| WO2003033729A1 |
Other References:
Biotechnology Letters,2005年,Vol.27,p963-968
Attorney, Agent or Firm:
Ikeuchi, Sato & Partners