サイトメガロウイルスエンハンサーおよびニワトリβ-アクチンプロモーターを含むハイブリッドプロモーターを利用したマイナス鎖RNAウイルスベクターの製造方法

Japanese Patent JP5438149

B2

The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken ²-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.

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Akihiro Iida
Hiroshi Ban
Makoto Inoue
Takahiro Hirata
Mamoru Hasegawa

JP2012028159A

March 12, 2014

February 13, 2012

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Dynabeck Research Institute Co., Ltd.

C12N15/09; A61K35/76; A61K39/155; A61K48/00; C12N5/10; C12N7/01; C12N7/02; C12N15/86

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Hatsushi Shimizu
Toshi Gobe
Koichi Niimi
Kazuya Kawamoto

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