Title:
単一細胞由来核酸の解析方法
Document Type and Number:
Japanese Patent JP6593710
Kind Code:
B2
Abstract:
The present invention relates to a method of analyzing a composition of nucleic acids derived from a single cell using a microplate including a plurality of reaction wells, the microplate having one bead arranged in one reaction well, the one bead having bound thereto a plurality of molecules of single-stranded oligonucleotides, the single-stranded oligonucleotides each having a nucleic acid capture sequence exposed at the 3′ end and a barcode sequence on the 5′ side of the nucleic acid capture sequence, the barcode sequence including a base sequence that differs from bead to bead.
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Inventors:
Shinichi Hashimoto
Shuichi Kaneko
Koji Matsushima
Shuichi Kaneko
Koji Matsushima
Application Number:
JP2016515907A
Publication Date:
October 23, 2019
Filing Date:
April 07, 2015
Export Citation:
Assignee:
idac Theranostics Co., Ltd.
International Classes:
C12M1/34; C12M1/00; C12N15/11; C12Q1/686; G01N33/50
Domestic Patent References:
| JP2010110262A | ||||
| JP2014506788A | ||||
| JP2002503954A | ||||
| JP2011036256A | ||||
| JP2005518819A | ||||
| JP2001509027A |
Foreign References:
| WO2012048341A1 | ||||
| WO2010087466A1 |
Other References:
Bio Techniques,2010年,Vol.48, No.5,p.409-412
Attorney, Agent or Firm:
Patent Service Corporation Tanigawa International Patent Office