METHOD FOR ANALYZING SINGLE NUCLEOTIDE REPEAT POLYMORPHISM AND SINGLE NUCLEOTIDE POLYMORPHISM

Title:

METHOD FOR ANALYZING SINGLE NUCLEOTIDE REPEAT POLYMORPHISM AND SINGLE NUCLEOTIDE POLYMORPHISM

Document Type and Number:

Japanese Patent JP2010246445

Kind Code:

A

Abstract:

To provide a method for analyzing the number of repeats of a single nucleotide repeat polymorphism and a single nucleotide polymorphism present near the single nucleotide repeat polymorphism by the use of genotyping markers.

The method for analyzing the number of repeats of a single nucleotide repeat polymorphism or a single nucleotide polymorphism includes the following steps: (a) a step of preparing genotyping markers which contain two or more sorts of labeled nucleic acid fragments corresponding to the nucleic acid region in which the target single nucleotide repeat polymorphism or a single nucleotide polymorphism near the single nucleotide repeat polymorphism is known to be present; (b) a step of preparing the test nucleic acid fragment by amplifying by the PCR method the corresponding region in the test nucleic acid to the nucleic acid fragment contained in the genotyping markers; (c) a step of mixing the genotyping markers and the test nucleic acid fragment and of subjecting the mixture to a separation treatment; and (d) the step of comparing the mobility of the test nucleic acid fragment with the mobility of nucleic acid fragments in the genotyping markers.

Inventors:

NISHIMORI KEI

Application Number:

JP2009098068A

Publication Date:

November 04, 2010

Filing Date:

April 14, 2009

Export Citation:

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Assignee:

NAT AGRICULTURE & FOOD RES ORG

International Classes:

C12Q1/68; C12N15/09

Domestic Patent References:

JP2003535567A	2003-12-02
JP2008517606A	2008-05-29
JP2001516594A	2001-10-02
JP2003535567A	2003-12-02
JP2008517606A	2008-05-29
JP2001516594A	2001-10-02

Foreign References:

WO2007119779A1	2007-10-25
WO2007119779A1	2007-10-25

Other References:

JPN6013048543; Ｊ．　Ｍｉｃｒｏｂｉｏｌ．　ＭｅｔｈｏｄｓＶｏｌ．７３，　Ｎｏ．３, 2008, ｐｐ．２６９-２７２
JPN6013048545; Ｊ．　Ｃｌｉｎ．　Ｍｉｃｒｏｂｉｏｌ．Ｖｏｌ．４４，　Ｎｏ．３, 2006, ｐｐ．７７７-７８２
JPN6013048546; Ｌｅｔｔ．　Ａｐｐｌ．　Ｍｉｃｒｏｂｉｏｌ．Ｖｏｌ．４６，　Ｎｏ．５, 2008, ｐｐ．６００-６０３
JPN6013048548; 結核Ｖｏｌ．８１，　Ｎｏ．１１, 2006, ｐｐ．６９７-７００
JPN6013048549; 動衛研研究報告Ｖｏｌ．１０９, 2003, ｐｐ．２５-３２
JPN6013048551; 結核Ｖｏｌ．８１，　Ｎｏ．９, 2006, ｐｐ．５５９-５６６
JPN6013048543; Ｊ．　Ｍｉｃｒｏｂｉｏｌ．　ＭｅｔｈｏｄｓＶｏｌ．７３，　Ｎｏ．３, 2008, ｐｐ．２６９-２７２
JPN6013048545; Ｊ．　Ｃｌｉｎ．　Ｍｉｃｒｏｂｉｏｌ．Ｖｏｌ．４４，　Ｎｏ．３, 2006, ｐｐ．７７７-７８２
JPN6013048546; Ｌｅｔｔ．　Ａｐｐｌ．　Ｍｉｃｒｏｂｉｏｌ．Ｖｏｌ．４６，　Ｎｏ．５, 2008, ｐｐ．６００-６０３
JPN6013048548; 結核Ｖｏｌ．８１，　Ｎｏ．１１, 2006, ｐｐ．６９７-７００
JPN6013048549; 動衛研研究報告Ｖｏｌ．１０９, 2003, ｐｐ．２５-３２
JPN6013048551; 結核Ｖｏｌ．８１，　Ｎｏ．９, 2006, ｐｐ．５５９-５６６

Attorney, Agent or Firm:

Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Nobuko Fukami

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