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Title:
METHOD FOR BIO-DENITRIFICATION OF HARDLY REMOVABLE AROMATIC ORGANIC NITROGEN COMPOUND
Document Type and Number:
Japanese Patent JP3243498
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To perform two phase denitrification reaction of petroleum org. solvent/water system for decomposing and removing different arom. org. nitrogen compd. with carbazole skeleton and quinoline skeleton with one kind of microorganism by bringing continuously a resting bacillus of a microorganism for decomposing an arom. org. nitrogen compd. into contact with a substance contg. the arom. org. nitrogen compd. and decomposing them.
SOLUTION: By using a bacterium isolated from the natural world by an accumulation cultivation method under a barely aerobic condition with an oxygen partial pressure condition being at most explosion limit and with assimilation and decomposition ability under an appropriate water content by using carbazole as a sole nitrogen source and a sole carbon source, quinoline which is another representative compd. with a skeleton as a hardly removable arom. compd. except carbazole is also assimilated and decomposed. From a view point for decomposing efficiently, e.g. the interface for bringing the denitrification bacterium into contact with the org. nitrogen compd. as a substrate is enlarged by using a surfactant and stirring appropriately the reaction system. As the result, the reaction in a petroleum fraction such as a crude oil and kerosene can be done in two phases of a petroleum org. solvent/water and a resting bacillus can be repeatedly used.


Inventors:
Ryuichiro Kurane
Akitsugu Usami
Kotaro Kirimura
Kenji Tsuji
Application Number:
JP5074497A
Publication Date:
January 07, 2002
Filing Date:
March 05, 1997
Export Citation:
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Assignee:
National Institute of Advanced Industrial Science and Technology
Petroleum Corporation
International Classes:
C12S1/00; C02F3/34; (IPC1-7): C02F3/34; C12S1/00
Domestic Patent References:
JP834981A
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)
Yusuke Hiraki (1 person outside)