Title:
METHOD OF CELL CULTURE
Document Type and Number:
Japanese Patent JPS611383
Kind Code:
A
Abstract:
PURPOSE: In subjecting a cell to suspension culture, to carry out the cultivation in an industrial scale in high density efficiently, by feeding a fluorocarbon having dissolved oxygen to a culture system.
CONSTITUTION: As a new medium is continuously fed from the line 1 to a system wherein a cell (preferably animal cell such as mouse × mouse hybridoma, etc.) is cultivated in a suspension state, an old culture solution is taken out from the line 4, and a fluorocarbon (preferably one liquid at normal temperature, such as perfluorodecalin, etc.) having dissolved oxygen is suspended as drops of liquid from the line 5 and recovered from the line 6, so that the culture is carried out while feeding oxygen.
Inventors:
ICHIKAWA YATAROU
HAMAMOTO KIMIHIKO
TOKASHIKI MICHIYUKI
HAMAMOTO KIMIHIKO
TOKASHIKI MICHIYUKI
Application Number:
JP12072484A
Publication Date:
January 07, 1986
Filing Date:
June 14, 1984
Export Citation:
Assignee:
TEIJIN LTD
International Classes:
B29C39/18; B29C45/14; C12N15/02; C12P21/08; H02K15/12; (IPC1-7): C12N5/02; C12N15/00
Domestic Patent References:
| JPS50125082A | 1975-10-01 | |||
| JPS50908A | 1975-01-08 |
Attorney, Agent or Firm:
Maeda Junhiro
Next Patent: PREPARATION OF N-ACETYLNEURAMINIC LYASE