METHOD FOR COLLECTING M-STAGE OR G1-STAGE CELL AND METHOD FOR TRANSPLANTING NUCLEUS USING THE CELL

Japanese Patent JP3842597

B2

PROBLEM TO BE SOLVED: To provide a method for collecting M-stage cell or G1-stage cell in a recovered cell population in which the ratio of cells in the M-stage or the G1-stage in a cell cycle is higher compared with that of a conventional method.
SOLUTION: The method for collecting the M-stage cells includes (1) a process for culturing mammal cells on a support in the presence of a cell-division metaphase synchronizing agent, (2) a process for shaking the support and collecting the cells separated from the support and (3) a process for collecting the cells having ≥20 μm diameter from the recovered cell population. The method for collecting the G1-stage cells includes (1) the process for culturing mammal cells on the support in the presence of the cell-division metaphase synchronizing agent, (2) the process for shaking the support and collecting the cells separated from the support, (3) a process for culturing the recovered cells in the absence of the cell-division metaphase synchronizing agent and (4) a process for collecting the cells having ≤15 μm diameter from the cultured cell population.

Urakawa truth
Aoyagi Keito

JP2001245948A

November 08, 2006

August 14, 2001

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Livestock fertilized egg transplantation technology research association

C12N5/00; C12N15/09; C12N5/02; C12N15/873; (IPC1-7): C12N15/09; C12N5/02

JP2001186827A

WO2001049106A1

Exp. Cell Res.,米国,1980年,Vol.126,397-405
Proc. Natl. Acad. Sci. USA,米国,1999年,Vol.96, No.26,14984-14989
Biochem. Biophys. Res. Commun.,米国,1996年,Vol.228,467-473

Eijiro Tanikawa

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