Title:
METHOD COMBINING IN SITU TARGET CAPTURE AND SPATIAL UNIQUE MOLECULAR IDENTIFIER (SUMI) IDENTIFICATION WITH IN VITRO SEQUENCING FOR HIGH DENSITY SPATIAL MULTIOMICS
Document Type and Number:
Japanese Patent JP2023155210
Kind Code:
A
Abstract:
To provide a method of combining in situ target capture and spatial unique molecular identifier (SUMI) identification with in vitro sequencing.SOLUTION: The present invention describes a method (High Density - SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ localization and identification (by in situ sequencing or sequential fluorescence hybridization) of rolonies derived from rolling circle amplification of circular oligonucleotides and in vitro sequencing of target captured RNA or DNA in combination with SUMI identification at a subcellular level with no optical diffraction limitation in the amount of captured target information that can be analyzed per cell. Apart from captured RNA or DNA, the High Density - SUMI-Seq method can also be applied using linear oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.SELECTED DRAWING: None
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Inventors:
THOMAS ROTHMANN
ROBERT PINARD
HOSONO MASAHIRO
ANDREAS BOSIO
ROBERT PINARD
HOSONO MASAHIRO
ANDREAS BOSIO
Application Number:
JP2023062354A
Publication Date:
October 20, 2023
Filing Date:
April 06, 2023
Export Citation:
Assignee:
MILTENYI BIOTEC BV & CO KG
International Classes:
C12Q1/6869; C12Q1/6806; C12Q1/686
Attorney, Agent or Firm:
Einzel Felix-Reinhard
Taku Morita
Junichi Maekawa
Hideo Nagashima
Ueshima class
Taku Morita
Junichi Maekawa
Hideo Nagashima
Ueshima class