METHOD AND COMPOSITION FOR RAPID MULTIPLEX AMPLIFICATION OF STR LOCI

Japanese Patent JP2017140042

A

PROBLEM TO BE SOLVED: To provide a method for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids.SOLUTION: According to the present invention, there is provided a method for detecting the presence of single nucleotide polymorphisms (SNPs) of a nucleic acid in a sample containing the nucleic acid. The method comprises: contacting in a solution the sample with eight or more unlabeled oligonucleotides under conditions permitting hybridization between the oligonucleotides and the nucleic acids in the sample; generating primer extension products; in the presence of a plurality of ddNTPs each labeled with one of eight or more different fluorescent dyes, labeling each product with the fluorescent dye by a primer extension reaction; and detecting the SNPs by detecting the products of the primer extension, each product labeled with the fluorescent dye by laser-induced fluorescence using a galvanometer in a step-and-stare mode and a spectrograph to detect the fluorescence from each of the fluorescent dyes.SELECTED DRAWING: None

JAMES W SCHUMM
SELDEN RICHARD F
EUGENE TAN

JP2017075347A

August 17, 2017

April 05, 2017

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NETBIO INC

C12Q1/68; G01N21/64

JP2002530121A	2002-09-17
JP2014515926A	2014-07-07
JP2014510510A	2014-04-24

US8137616B2	2012-03-20
US20110183857A1	2011-07-28
US20090059222A1	2009-03-05
US8018593B2	2011-09-13
US20050280817A1	2005-12-22

Mitsuo Tanaka
Hiroshi Yamazaki
Tsuyoshi Sato

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