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Title:
METHOD FOR DETECTING CARBOHYDRATE UTILIZING PH INDICATOR
Document Type and Number:
Japanese Patent JP2005127759
Kind Code:
A
Abstract:

To find a method for surely and easily detecting carbohydrate of a CD, a starch portion degraded material, or the like, and to develop a means of detecting CGTase, starch degradative enzyme, and their produced germ.

The method is used to detect carbohydrate of the CD, the starch degraded material, and the like in a sample to be inspected, according to coloring changes, by adding a buffer solution and pH indicator at a prescribed pH region to the sample to be inspected. In test paper for detecting cyclodextrin and/or the starch portion degraded material, the combination of the buffer solution at a specified pH region and the pH indicator is made to be contained in a filter paper. In CGTase and/or starch portion degradative enzyme or the medium for detecting CD and/or the starched portion degraded material, the combination of the buffer solution at a prescribed pH region and the pH indicator, soluble starch or starch, a medium for cultivating cyclodextrin synthesized enzyme or a microbe, having a starch portion degradative enzyme production capability, and agar or gellan gum are mixed.


Inventors:
KOBAYASHI SHOICHI
MIKUNI KATSUHIKO
HARA KOZO
KUWABARA NOBUHIRO
Application Number:
JP2003361433A
Publication Date:
May 19, 2005
Filing Date:
October 22, 2003
Export Citation:
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Assignee:
YOKOHAMA KOKUSAI BIO KENKYUSHO
International Classes:
G01N31/00; C12N1/00; C12Q1/04; G01N21/77; G01N21/78; G01N31/22; (IPC1-7): G01N31/00; C12Q1/04; G01N21/77; G01N21/78; G01N31/22
Attorney, Agent or Firm:
Fujiro Kubota
Yuya Yano