Title:
METHOD OF DETECTING COMPLEMENTARY STRAND NUCLEIC ACID MOLECULE SEQUENCE AND METHOD OF DETECTING SNP USING π-CONJUGATION TYPE ELECTROCHEMICALLY ACTIVE NONNATURAL NUCLEOSIDE
Document Type and Number:
Japanese Patent JP2006182757
Kind Code:
A
Abstract:
To provide a new DNA probe which shows a very large difference in the electrochemical response between a perfect complementary strand and SNP and can obtain an almost digital on/off response, and a method of detecting a complementary strand nucleic acid molecule sequence and a method of detecting SNP which use the probe.
A nonnatural nucleoside constituted of an electrochemically active site and nucleic acid bases which are bound via a spacer through which π-electrons can conjugate or its phosphoramidite derivative is provided, and the DNA probe contains the same.
Inventors:
INOUE MASAHIKO
CHIBA JIYUNYA
IKEDA REONA
TAKASE MASAYOSHI
CHIBA JIYUNYA
IKEDA REONA
TAKASE MASAYOSHI
Application Number:
JP2005218129A
Publication Date:
July 13, 2006
Filing Date:
July 28, 2005
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY
International Classes:
C07H23/00; C12M1/00; C12N15/09; C12Q1/68; G01N27/416; G01N33/53; G01N33/566
Domestic Patent References:
| JP2004526409A | 2004-09-02 | |||
| JP2002513592A | 2002-05-14 |
Foreign References:
| WO2004035829A1 | 2004-04-29 | |||
| WO2003074731A2 | 2003-09-12 |
Attorney, Agent or Firm:
Masahiro Abe