Title:
METHOD FOR DETECTING AND DIFFERENTIATING ADENOVIRUS AND OLIGONUCLEOTIDE AND DNA FRAGMENT USED THEREFOR
Document Type and Number:
Japanese Patent JP3709211
Kind Code:
B2
Abstract:
PURPOSE: To detect and differentiate the subject virus rapidly and simply by amplifying a base sequence part specific to a subgroup of a gene region encoding a hexon of adenovirus and to a blood group with a primer complementary to a sequence common to a blood group.
CONSTITUTION: A part having a size of 1263 base pairs among 1897th to 2907th base pairs of a gene region encoding a hexon of adenovirus as a part having a base sequence part specific to a subgroup of the gene region and to a blood group is amplified by using two kinds of oligonucleotides complementary to a base sequence common to the blood group in the gene region as primers at 5' end side and 3' end side. An oligonucleotide having a base sequence preferably to a subgroup in the gene region encoding the hexon of adenovirus and to a blood group is labeled and the amplified DNA is hybridized as a DNA probe. The kind of the hybridized DNA probe is analyzed to surely, rapidly and simply detect adenovirus and to differentiate its subgroup and blood group.
Inventors:
Akira Oshima
Hiroaki Ishiko
Hiroaki Ishiko
Application Number:
JP12616394A
Publication Date:
October 26, 2005
Filing Date:
June 08, 1994
Export Citation:
Assignee:
Mitsubishi Kagaku BCS Co., Ltd.
International Classes:
C12N15/09; C07H21/04; C07K14/075; C12Q1/68; C12Q1/70; (IPC1-7): C12Q1/68; C12N15/09; C12Q1/70
Attorney, Agent or Firm:
Hajime Tsukuni
Mutsuo Watanabe
Mutsuo Watanabe
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