METHOD FOR DETECTING MUTATION AND KIT USED IN THE SAME

Japanese Patent JP2012040029

A

To provide a method for detecting a mutation that uses Tm analysis and is excellent in detection sensitivity.

A detection probe consisting of a polynucleotide complementary to a target sequence to be detected containing a detection site that has been mutated, and an inhibitory polynucleotide complementary to the non-target sequence not to be detected containing the detection site that is unmutated are added to a sample containing a DNA to be detected in which the detection site has been mutated and a DNA not to be detected in which the detection site is unmutated, so that the detection probe is hybridized by the DNA. Then while the hybrid product between the DNA and the detection probe is heated, a signal variation associated with rise in temperature is measured, then the signal variation is analyzed, and thereby a Tm value is determined, so that the presence of the mutation is determined.

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HIRAI MITSUHARU
MAJIMA TOMOJI
MAEKAWA TAIRA
KIMURA SHINYA

JP2011261298A

March 01, 2012

November 30, 2011

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ARKRAY INC

C12Q1/68; C12N15/09

JP2003038180A	2003-02-12
JP2004527221A	2004-09-09
JP2004537992A	2004-12-24
JP2005520554A	2005-07-14
JP2004524032A	2004-08-12
JP2004534203A	2004-11-11
JP2005524384A	2005-08-18

WO2004111072A2

2004-12-23

Koichiro Tsujimaru
Yumi Nakayama
Reiko Yoshida
Isaji So

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