Title:
METHOD FOR DETECTING VASCULAR ENDOTHELIAL CELLS THAT INHIBIT GROWTH OF VASCULAR SMOOTH MUSCLE CELLS
Document Type and Number:
Japanese Patent JP2014233217
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for detecting "good vascular endothelial cells" having an inhibitory effect on the growth of vascular smooth muscle cells, and to provide a therapeutic technology of transplanting the "good vascular endothelial cells" to the arterial inner surface.SOLUTION: The inventor has identified a causative gene RGS5 (Regulator of G-protein signaling 5) that lowers inhibitory action of vascular endothelial cells onto the growth of vascular smooth muscle cells. Thus, by checking the expression of mRNA or protein of RGS5, it becomes possible to identify "good vascular endothelial cells" as the RGS5-negative vascular endothelial cells in a short time without culture facilities. Thus obtained "good endothelial cells" can be efficiently supplied to an arterial inner surface where the endothelial cells have been stripped by using "per-vasa vasorum transplantation" technology to restore the vascular structure. For example, the "good vascular endothelial cells" embedded in a substrate material that becomes gel in the body may be put on the blood vessel wall of the artery subjected to PCI treatment etc.
Inventors:
SAEKI KUMIKO
YUO AKIRA
NISHIO MIWAKO
SAEKI KOICHI
HASEGAWA MAMORU
YUO AKIRA
NISHIO MIWAKO
SAEKI KOICHI
HASEGAWA MAMORU
Application Number:
JP2013115325A
Publication Date:
December 15, 2014
Filing Date:
May 31, 2013
Export Citation:
Assignee:
NAT CT FOR GLOBAL HEALTH & MEDICINE
DNAVEC CORP
DNAVEC CORP
International Classes:
C12Q1/04; A61K35/12; C12N5/071; C12N5/10; C12Q1/68; G01N33/15; G01N33/50; G01N33/53
Domestic Patent References:
| JP2010131373A | 2010-06-17 |
Other References:
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BONDJERS, C. ET AL., AM. J. PATHOL., vol. 162, no. 3, JPN6017003162, March 2003 (2003-03-01), pages 721 - 729, ISSN: 0003491007
CHEN, X. ET AL., MOD. PATHOL., vol. 17, no. 10, JPN6017003164, October 2004 (2004-10-01), pages 1198 - 1210, ISSN: 0003491008
SEKI, N. ET AL., J. HUM. GENET., vol. 43, no. 3, JPN6017034064, 1998, pages 202 - 205, ISSN: 0003637219
佐藤千香子、外８名: "「ヒト胚性幹細胞由来血管内皮細胞および末梢血単球由来血管内皮細胞による血管平滑筋細胞の増殖抑制」", 再生医療, vol. 9, JPN6017003166, 5 February 2010 (2010-02-05), pages 173, ISSN: 0003749103
中原正子、外５名: "「血管内皮細胞による血管平滑筋増殖抑制の機序：動脈狭窄症の新規治療開発に向けて」", 再生医療, vol. 11, JPN6017003168, 16 May 2012 (2012-05-16), pages 167, ISSN: 0003749104
ARNOLD, C. ET AL., EMBO MOLECULAR MEDICINE, vol. 6, JPN6017003159, 27 June 2014 (2014-06-27), pages 1075 - 1089, ISSN: 0003749105
Attorney, Agent or Firm:
Masao Haruna
Spring water Original aim
Hirotaka Yamaguchi
Shun Osakabe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Shin-ichi Watanabe
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Spring water Original aim
Hirotaka Yamaguchi
Shun Osakabe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Shin-ichi Watanabe
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto