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Title:
METHOD AND DEVICE FOR ANALYZING SAMPLE CONTAINING A PLURALITY OF FLUORESCENT MATERIALS
Document Type and Number:
Japanese Patent JP3915651
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To provide a method and a device capable of precisely carrying out an analysis with less noise when analyzing a plurality of fluorescent materials by using a white continuous spectral light pulse obtained from an ultra-short light pulse.
SOLUTION: In the method, intensity modulation processes are carried out for exciting light λ1,λ2 and λ3 corresponding to the fluorescent materials to be targeted at respective frequencies f1, f2 and f3, and then composite light pulses composed of modulated light are projected onto a sample 21, thereby exciting the target fluorescent materials, and a spectral process is carried out for excited fluorescent light at wavelength values η1,η2 and η3 of the target fluorescent materials, and outputs of respective spectral fluorescent light at the frequencies f1, f2 and f3 are synchronously rectified by using a fluorescent detecting system 22. For example, the intensity of the fluorescent light with the wavelength η1 is varied at the frequency f1 because of corresponding to the exciting light with the wavelength λ1. Therefore, the output s11 from a rectifier c11 after carrying out the synchronous rectification at the frequency f1 contains only the fluorescent light from the target fluorescent material.


Inventors:
Kunihiko Okubo
Junichi Kita
Kinoshita Taisei
Hiroshi Nakano
Ito Ichiyoshi
Fukui Kiichi
Application Number:
JP2002295739A
Publication Date:
May 16, 2007
Filing Date:
October 09, 2002
Export Citation:
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Assignee:
SHIMADZU CORPORATION
International Classes:
G01N21/64; (IPC1-7): G01N21/64
Domestic Patent References:
JP8506419A
JP2001066253A
JP62192632A
JP9113240A
JP2003172702A
JP11507143A
Attorney, Agent or Firm:
Ryouhei Kobayashi