Title:
METHOD FOR DISCRIMINATE TARGET BASE OF DNA AND ALLELE-SPECIFIC PRIMER USED THEREOF
Document Type and Number:
Japanese Patent JP2007135540
Kind Code:
A
Abstract:
To obtain an allele-specific primer in which when a base directly adjacent to the 3' side of a target SNP base is T and a base adjacent to the base is G, possibility of false positiveness is extremely low and clear SNP discrimination is possible.
The method for discriminating a target base of DNA is designed such that a 3' terminal base is an SNP corresponding base, the second base from the 3' terminal is G, the third base from the 3' terminal is G or T and the base sequence from the fourth base from the 3' terminal to the 5' terminal base is complementary to a base sequence from the target SNP base to a desired base from a base adjacent to three bases to the 3' side.
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Inventors:
YAKU EISHIN
OKA HIROAKI
YUKIMASA TETSUO
OKA HIROAKI
YUKIMASA TETSUO
Application Number:
JP2005337606A
Publication Date:
June 07, 2007
Filing Date:
November 22, 2005
Export Citation:
Assignee:
MATSUSHITA ELECTRIC IND CO LTD
International Classes:
C12N15/09; C12Q1/68; G01N33/53
Attorney, Agent or Firm:
Yoshihiro Tsunoda
Yasu Furukawa
Toshio Nishitani
Keiji width
Yasu Furukawa
Toshio Nishitani
Keiji width
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