Title:
METHOD OF HEATING SAMPLE, AND MICROCHIP
Document Type and Number:
Japanese Patent JP2015062360
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a technique allowing for suppression of evaporation or air bubbles generation in a liquid sample in a microchip during heating, with a simpler constitution.SOLUTION: A microchip 1 has a substrate 2, a protective member 3, a reaction vessel (sealed space) 5, and a gas generating film (gas generating agent) 6. The gas generating film 6 is comprised of a photoresponsive or thermal responsive gas generating agent. In heating a liquid sample 10 housed in the reaction vessel 5, external stimulation, such as light, is given to the gas generating film 6 to generate gas. Thus, pressure by gas is applied to the reaction vessel 5, and a liquid sample 10 can be heated while pressure is applied thereto. A heating method of the present invention can be used in a degeneration (dissociation) process or the like in nucleic acid amplification reaction.
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Inventors:
AKAGI YOSHINORI
YAMAMOTO KAZUKI
MATSUMOTO SHUICHIRO
NOMURA SHIGERU
YAMAMOTO KAZUKI
MATSUMOTO SHUICHIRO
NOMURA SHIGERU
Application Number:
JP2013197664A
Publication Date:
April 09, 2015
Filing Date:
September 25, 2013
Export Citation:
Assignee:
SEKISUI CHEMICAL CO LTD
International Classes:
C12Q1/68; B01J7/00; B01J19/00; B81B1/00; C09J7/00; C09J11/06; C09J201/02; C12M1/00; C12N15/09; G01N35/00; G01N37/00
Domestic Patent References:
| JP2003299485A | 2003-10-21 | |||
| JP2012188306A | 2012-10-04 | |||
| JP2012072007A | 2012-04-12 | |||
| JP2012062238A | 2012-03-29 | |||
| JP2004195584A | 2004-07-15 | |||
| JP2011030522A | 2011-02-17 | |||
| JP2006214494A | 2006-08-17 |
Foreign References:
| US20130121893A1 | 2013-05-16 | |||
| WO2007055151A1 | 2007-05-18 |
Attorney, Agent or Firm:
Takashi Fujita