Title:
METHOD FOR IMPROVING EFFICIENCY OF NUCLEIC ACID HYBRIDIZATION
Document Type and Number:
Japanese Patent JP2001252097
Kind Code:
A
Abstract:
To provide a method for improving the efficiency of hybridization by preventing the formation of a three-dimensional structure formed by a hydrogen bond in a nucleic acid molecule.
This method for detecting a base sequence of a probe nucleic acid and a complementary sequence in the nucleic acid in a sample, comprising hybridization of the nucleic acid in the sample with the probe nucleic acid is characterized in that one or more kinds of bases in the nucleic acid in the sample and/or the probe nucleic acid are previously chemically modified.
Inventors:
SUDO YUKIO
SHINOKI HIROSHI
OOKUBO KOUSAKU
SHINOKI HIROSHI
OOKUBO KOUSAKU
Application Number:
JP2000065862A
Publication Date:
September 18, 2001
Filing Date:
March 10, 2000
Export Citation:
Assignee:
FUJI PHOTO FILM CO LTD
International Classes:
C12N15/09; C12Q1/68; G01N37/00; G01N33/53; (IPC1-7): C12Q1/68; C12N15/09; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Masazumi Imamura (2 outside)
Previous Patent: JP2001252096
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