Title:
標的核酸への変異の導入方法
Document Type and Number:
Japanese Patent JP4372684
Kind Code:
B2
Abstract:
A method of transferring a mutation into the base sequence of a target nucleic acid characterized by comprising the step of preparing a DNA having a reversed repetitive sequence wherein the base sequence of the DNA having the reversed repetitive sequence is homologous with the target nucleic acid and has a base sequence containing the mutation to be transferred into the target nucleic acid, and the step of transferring the DNA having the reversed repetitive sequence into cells; and a kit for the method.
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Inventors:
Cao Chun
Hiroaki Sagawa
Ikunoshin Kato
Hiroaki Sagawa
Ikunoshin Kato
Application Number:
JP2004521183A
Publication Date:
November 25, 2009
Filing Date:
July 11, 2003
Export Citation:
Assignee:
Takara Bio Inc.
International Classes:
C12N15/09; C07K14/435; C12N15/10; C12N15/90
Domestic Patent References:
| JP5308972A |
Foreign References:
| WO2001049844A1 |
Other References:
J Virol, 73(11), p.9303-9313 (1999)
Biochim Biophys Acta, 1071, p.83-101 (1991)
Genetics, 153, p.1873-1883 (1999)
Nucleic Acids Res, vol.29, no.12, e55 (2001)
Biochim Biophys Acta, 1071, p.83-101 (1991)
Genetics, 153, p.1873-1883 (1999)
Nucleic Acids Res, vol.29, no.12, e55 (2001)
Attorney, Agent or Firm:
Mitsuo Tanaka
Michiko Matsutani
Tadayuki Motoyama
Kenji Tomita
Michiko Matsutani
Tadayuki Motoyama
Kenji Tomita