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Title:
METHOD FOR ISOLATING MULTIPLE-DRUG-RESISTANT PSEUDOMONAD AERUGINOSA (MDRP) AND SELECTIVE CULTURE MEDIUM FOR MULTIPLE-DRUG-RESISTANT PSEUDOMONAD AERUGINOSA
Document Type and Number:
Japanese Patent JP2009159837
Kind Code:
A
Abstract:

To provide a method for quickly isolating multiple-drug-resistant Pseudomonas aeruginosa (MDRP) without causing an omission in picking-up, and to provide a selective culture medium therefor.

Provided are the method for isolating MDRP, comprising dissolving 35.8 g of a NAC agar culture medium (dried powder), 16 mg of ciprofloxacin hydrochloride, and 32 mg of amikacin in 1,000 mL of water, boiling the solution, cooling the boiled solution to 60C, adding 0.032 g of an imipenem cilastatin sodium equivalent weight mixture to make an agar plate culture medium, smearing the culture medium with a sample such as a patient specimen, culturing it at a constant temperature of 361C for 24 hours, and checking the presence or absence of a yellow to yellowish green colony on the culture medium; and the selective culture medium.


Inventors:
YAMAZAKI MIKAKO
KATSUMATA KOHEI
KOYAMA TOMONORI
FUJIOKA TAKAHIRO
Application Number:
JP2007339880A
Publication Date:
July 23, 2009
Filing Date:
December 28, 2007
Export Citation:
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Assignee:
TECH RES DEV INST MINI DEFENCE
International Classes:
C12N1/02; C12N1/20
Domestic Patent References:
JP2002159447A2002-06-04
JP2003510263A2003-03-18
Attorney, Agent or Firm:
Takashi Murai