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Title:
METHOD FOR ISOLATING RNA
Document Type and Number:
Japanese Patent JP2004329176
Kind Code:
A
Abstract:

To provide a method for isolating an RNA quickly and simply.

This method for isolating the RNA comprises a process of extracting the RNA with an organic solvent such as phenol, chloroform, etc., by suspending a specimen in an aqueous solution containing a salt and a buffering agent under an acid condition, and a recovering process of the RNA contained in an aqueous solution by an alcohol precipitation method. Since the method can obtain a high purity RNA in a small number of steps simply and rapidly, it is suitable for the simultaneous treatments of many specimens and a mechanization. Also, since the specimen is not treated with SDS (sodium dodecylsulfate), guanidine thiocyanate, etc., it is possible to isolate the RNA in a high degree of purification even from a biological specimen containing much starch. Therefore, the method is suitably used for the extraction of the RNA from a plant tissue containing much starch such as a seed, etc.


Inventors:
KADONA RISA
Application Number:
JP2003133633A
Publication Date:
November 25, 2004
Filing Date:
May 12, 2003
Export Citation:
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Assignee:
PLANT GENOME CT CO LTD
International Classes:
C12N15/09; C12M1/00; (IPC1-7): C12N15/09; C12M1/00
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto