Title:
ポリエチレングリコール系非イオン性界面活性剤を用いた菌体の死滅化方法
Document Type and Number:
Japanese Patent JP5926818
Kind Code:
B2
Abstract:
The present invention relates to a method for sterilizing microbial cells and to sterilized microbial cells, in which microbial cells or a culture containing microbial cells is treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. In detail, the present invention relates to a method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant that can be used for foods so that the microbial cells are sterilized to be used in food production. Further, the present invention relates to a material containing sterilized microbial cells that can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.
Inventors:
Kim, Jinha
Lee, Young Mi
Kim, Sombo
Kim, tech bomb
Kim, Yang Hee
Park, Seung Won
Lee, Young Mi
Kim, Sombo
Kim, tech bomb
Kim, Yang Hee
Park, Seung Won
Application Number:
JP2014548660A
Publication Date:
May 25, 2016
Filing Date:
December 20, 2012
Export Citation:
Assignee:
CJ Cherjedan Dan Corporation
International Classes:
A23L29/00; C12P19/02; A01P3/00; C12N1/20; C12N9/90
Domestic Patent References:
| JP2010510774A |
Foreign References:
| WO2001036592A1 | ||||
| US3912450 |
Attorney, Agent or Firm:
Ryu Iwatani