METHOD FOR LABELING OR DETECTING SUBSTANCE BY USING LUMINESCENT ARYL SULFONATE CYANINE DYE

Title:

METHOD FOR LABELING OR DETECTING SUBSTANCE BY USING LUMINESCENT ARYL SULFONATE CYANINE DYE

Document Type and Number:

Japanese Patent JP2898264

Kind Code:

B2

Abstract:

PROBLEM TO BE SOLVED: To provide a luminescent light-stable reaction product useful for the investigation of labeling by covalently reacting the group, such as amine or sulfhydryl, of a component to be labeled, comprising, e.g. protein or viruses with a sulfoindolenine-based polymethine dye.
SOLUTION: A luminescent (fluorescent) reaction product concerning the investigation of labeling of proteins, nucleic acids, DNAs, microorganisms, polymers, etc., and obtained through the covalent reaction of a luminescent polymethine cyanine or related polymethine dye with the amine, hydroxyl, etc., of the above-mentioned materials. It is a reaction product of a component having groups such as amine, hydroxyl (and aldehyde or sulfhydryl by using an antibody-antigen reaction) with a (water-soluble) sulfoindolenine-based or naphthosulfoindolenine-based polymethine dye having substituent(s) to be reacted with the groups of the component to form a covalent bond, wherein the molecule of the dye on the reaction product has an average molecular extinction coefficient of at least 50,000l/mol.cm and an average quantum absorption of at least 2% and absorbs and radiates a light with a maximum wavelength of 400-850nm.

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Inventors:

ARAN ESU WAGONAA

Application Number:

JP24700397A

Publication Date:

May 31, 1999

Filing Date:

September 11, 1997

Export Citation:

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Assignee:

KAANEGII MERON UNIV

International Classes:

G01N21/64; C09B23/00; C09B23/01; C09B23/02; C09B23/10; C09B23/14; C09B62/002; C09K11/06; C12P21/04; G01N21/77; G01N21/78; G01N33/483; G01N33/50; G01N33/53; G01N33/532; G01N33/533; G01N33/569; G01N33/58; (IPC1-7): C09B23/00; G01N33/533; G01N33/58

Attorney, Agent or Firm:

Kazuo Shamoto (5 outside)

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