METHOD OF MAKING FIXED ENZYME, MICROORGANISM OR ORGANELLE

Title:

METHOD OF MAKING FIXED ENZYME, MICROORGANISM OR ORGANELLE

Document Type and Number:

Japanese Patent JPS5539719

Kind Code:

A

Abstract:

PURPOSE: A carrier resulting from immersing a PVC resin solution into a solvent that is poor compatible with PVC and high with the solvent of the solution is brought into contact with a solution or dispersion containing enzyme, thus fixing a large amount of enzyme of high activity.

CONSTITUTION: A PVC resin is dissolved in a solvent A consisting of at least one as DMF to form a solution of 2W25wt% resin concentration. The solution is casted on a base or sprayed into the air to form a desired carrier of membrane or particles. When the solution visually whitens after the formation into the film or particles, it is immersed into another solvent B that is less compatible to PVC and high compatible to solvent A as water or methanol to give the soldified carrier. The resulting carrier is brought into contact with a solution containing an enzyme in 1W200mg/dl concentration to make said fixed enzyme. Resultingly, fixed enzymes of high activity is obtained in a short time.

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Inventors:

SUZUKI SHIYUUICHI
HIROSE YUKIO
HAYASHI MITSUHIRO

Application Number:

JP11172978A

Publication Date:

March 19, 1980

Filing Date:

September 13, 1978

Export Citation:

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Assignee:

MITSUBISHI PETROCHEMICAL CO

International Classes:

C12N11/08; C07K17/08; C08J3/14; C08J5/18; (IPC1-7): C12N11/08

Previous Patent: JPS5539718

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