Title:
METHOD FOR MAKING INSERTIONAL MUTATION
Document Type and Number:
Japanese Patent JP2013165731
Kind Code:
A
Abstract:
To provide a method for making insertional mutations at random or quasi-random locations in the cellular nucleic acid of a target cell.
This method for making insertional mutations at random or quasi-random locations in the cellular nucleic acid of a target cell includes a step of introducing a synaptic complex to the target cell under conditions that mediate transpositions into the cellular nucleic acid, where the synaptic complex comprises: (a) a Tn5 transposase protein and (b) a polynucleotide that comprises a pair of nucleotide sequences adapted for operably interacting with Tn5 transposase to form the synaptic complex and a transposable nucleotide sequence therebetween.
Inventors:
REZNIKOFF WILLIAM S
GORYSHIN IGOR Y
GORYSHIN IGOR Y
Application Number:
JP2013100415A
Publication Date:
August 29, 2013
Filing Date:
May 10, 2013
Export Citation:
Assignee:
WISCONSIN ALUMNI RES FOUND
International Classes:
C12N15/09; C12N9/00; G01N33/50; C12N9/22; C12N15/00; C12N15/01; C12N15/10; C12N15/90; C12Q1/02; G01N33/15; G01N33/566
Domestic Patent References:
| JP2010068813A | 2010-04-02 |
Foreign References:
| WO1998010077A1 | 1998-03-12 |
Other References:
JPN6012025281; ＴＨＥ ＪＯＵＲＮＡＬ ＯＦ ＢＩＯＬＯＧＩＣＡＬ ＣＨＥＭＩＳＴＲＹ ｖｏｌ．２７３ ｎｏ．１３, 199803, ｐｐ．７３６７-７３７４
JPN6012025281; ＴＨＥ ＪＯＵＲＮＡＬ ＯＦ ＢＩＯＬＯＧＩＣＡＬ ＣＨＥＭＩＳＴＲＹ ｖｏｌ．２７３ ｎｏ．１３, 199803, ｐｐ．７３６７-７３７４
JPN6012025281; ＴＨＥ ＪＯＵＲＮＡＬ ＯＦ ＢＩＯＬＯＧＩＣＡＬ ＣＨＥＭＩＳＴＲＹ ｖｏｌ．２７３ ｎｏ．１３, 199803, ｐｐ．７３６７-７３７４
Attorney, Agent or Firm:
Koichi Tsujii
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Takamitsu Hoshino
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Takamitsu Hoshino