To provide a method of simply and inexpensively manufacturing a useful concentrate of a trypsin inhibitor and the like contained in soybeans and having a physiological activity.

In the manufacturing method of the soybean trypsin inhibitor cocentrate, a soybean whey is concentrated to 10 to 30 wt.% in solid content concentration, 3 wt.% or more of a salt is added to the concentrated soybean whey to recover agglomerated precipitate precipitated under an acid condition.