Title:
METHOD AND MEANS FOR ISOLATION AND PURIFICATION OF NUCLEIC ACID ON SURFACE
Document Type and Number:
Japanese Patent JP2008220377
Kind Code:
A
Abstract:
To provide new methods and means for isolation and purification of a nucleic acid on the surface.
A method using the surface, e.g., a porous film, immobilizing with a simple method a nucleic acid from a sample including the nucleic acid, also releasing with a simple process and thereby specifically enabling practice of a perfectly automated method, is provided. And further, in the subsequent reaction process, the nucleic acid is released from the phase without complexity again and the nucleic acid is used, e.g., for restriction digestion, RT, PCR, or RT-PCR or in another analytical or enzymatic reaction. A specific isolation vessel performing the method is used.
Inventors:
GAUCH SIMONE
BASTIAN HELGE
ULLMANN SUSANNE
OELMUELLER UWE
WEBER MARTIN
FUHRMANN GUIDO
SCHORR JOACHIM
BASTIAN HELGE
ULLMANN SUSANNE
OELMUELLER UWE
WEBER MARTIN
FUHRMANN GUIDO
SCHORR JOACHIM
Application Number:
JP2008101052A
Publication Date:
September 25, 2008
Filing Date:
April 09, 2008
Export Citation:
Assignee:
QIAGEN GMBH
International Classes:
C12N15/09; C07H1/08; C12M1/00; C12Q1/68; C12Q1/6844
Domestic Patent References:
| JPH04325099A | 1992-11-13 | |||
| JPH04187077A | 1992-07-03 | |||
| JPH0515373A | 1993-01-26 | |||
| JPH02295484A | 1990-12-06 | |||
| JPH0310686A | 1991-01-18 | |||
| JPH05268963A | 1993-10-19 |
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes