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Title:
METHOD FOR MEASURING ATP IN LIQUID
Document Type and Number:
Japanese Patent JP2013005765
Kind Code:
A
Abstract:

To provide a method for measuring ATP for correcting a unique value, such as permeability, color or pH, possessed by the liquid component.

The method for measuring an amount of ATP in a sample as a liquid includes steps of: extracting ATP (S103) existing in microorganism cells contained in the sample after erasing the ATP (S102) existing in the microorganism cells contained in the sample; adding a luminescence reagent and measuring an amount of a first light emission (S104) after adding the predetermined ratio of ATP standard solution containing the known amount of the ATP; adding the luminescence reagent and measuring an amount of a second light emission (S105) after adding the predetermined ratio of blank liquid containing less amount of the ATP than the ATP standard solution; and calculating an amount of the ATP (S106) in the sample based on the amounts of the first light emission and the second light emission.


Inventors:
NAKATSUKA YUTA
MIYASHITA NOE
MORI SHUICHI
Application Number:
JP2011141142A
Publication Date:
January 10, 2013
Filing Date:
June 24, 2011
Export Citation:
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Assignee:
HITACHI PLANT TECHNOLOGIES LTD
International Classes:
C12Q1/06; G01N21/76; C12Q1/66; G01N33/14
Domestic Patent References:
JPH09182600A1997-07-15
JP2000189197A2000-07-11
JP2001136999A2001-05-22
Other References:
JPN6015006057; Clin. Chem., 2007, 53(2), pp.318-325
JPN6015006060; Luminescence, 2003, 18(3), pp.173-181
Attorney, Agent or Firm:
Isono Dozo
Etsuo Tada