Title:
フラビン結合型グルコースデヒドロゲナーゼを用いたグルコースの測定方法
Document Type and Number:
Japanese Patent JP7109155
Kind Code:
B2
Abstract:
To accurately measure the amount of 10 mM or less of D-glucose.SOLUTION: A D-glucose is measured by contacting a flavin-binding type GDH with the D-glucose, at 20-40°C, wherein the flavin-binding type GDH has (i) an action: exhibiting GDH activity under the presence of an electron acceptor, (ii) a substrate specificity: having a less reactivity to maltose, D-galactose, and D-xylose as compared with that to D-glucose, (iii) a thermal stability: having 80% or more of a residual activity after a heat treatment at 40°C for 15 minutes, (iv) having a flavin compound as a coenzyme, and (v) a temperature characteristic: having an active value of 74-100% at 20-40°C when the reactivity to D-glucose at a measurement temperature with the highest activity in the range of 20-40°C is 100%.SELECTED DRAWING: None
Inventors:
鉞 陽介
Application Number:
JP2016111857A
Publication Date:
July 29, 2022
Filing Date:
June 03, 2016
Export Citation:
Assignee:
Kikkoman Corporation
International Classes:
C12Q1/32; C12N15/53; G01N33/66
Domestic Patent References:
| JP4648993B2 | ||||
| JP2013176363A |
Foreign References:
| WO2013065623A1 | ||||
| WO2013118798A1 |
Other References:
糖尿病,2002年,Vol.45,p.821-824
岩波 生物学辞典 第4版,1997年4月10日,株式会社 岩波書店発行,400頁「血糖」の項
岩波 生物学辞典 第4版,1997年4月10日,株式会社 岩波書店発行,400頁「血糖」の項
Attorney, Agent or Firm:
Hiraki International Patent Office