To provide a means for accurately measuring the number of pollutant-degrading microbes existing in polluted soil or the like.
This method for measuring the number of the pollutant-degrading microbes comprises the following processes. (1) A process for stepwisely diluting a sample gathered from an environment. (2) A process for preparing a plurality of liquid culture medium-containing containers at a dilution step, adding a diluted sample and a pollutant labeled with a stable isotope to each container, and culturing the sample at each culture step. (3) A process for measuring a stable isotope-containing substance after the culture and examining the presence or absence of the pollutant-degrading microbes in the sample on the basis of the measurement result. (4) A process for statistically estimating the number of the pollutant-degrading microbes in the original sample from the dilution step of the sample in which the presence of the pollutant-degrading microbes was judged and from the number of the pollutant-degrading microbes.
KOIKE HIROMITSU
AOYAMA KATSUHIRO
Setuko Mayama
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