To provide a method for accurately detecting and measuring in a short time even a very small amount of substance to be detected such as a chemical substance, a protein, a microbe, viruses, etc., capable of becoming an antigen and an antibody.
An area for fixing a substance for capturing the substance to be detected in which a first substance of 0.002-0.8 m2 in size for capturing the substance to be detected is fixed is formed on a substrate. The substance to be detected contained in a sample liquid is captured in the area for fixing the substance for capturing the substance to be detected. After a second substance for capturing the substance to be detected labeling an enzyme is combined with the substance to be detected, a substance to be a substrate for an enzyme reaction is added to create an enzyme reaction product. An electrochemical reaction caused in a process of the progress of the enzyme reaction is detected as an electric signal by a working electrode provided correspondingly in the vicinity of the area for fixing the substance for capturing the substance to be detected.
ONOUCHI TORU
NIWA KAZUHIRO
SUZUKI MASATO
IBAYASHI MASARU
TERAYAMA MASANORI
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