To provide a method for obtaining a number of amplification products of nucleic acids at one time from a pair of primer sets when a nucleic acid is amplified through applying the PCR method in a method for detecting nucleic acid mutation which detects the nucleic acid mutation from a dissociation curve of a target double strand nucleic acid to a single strand or a dissociation pattern.

A number of the amplification products of the target nucleic acids are obtained by performing the PCR method adding a pair of or several pairs of primers having a structure and a corresponding structure to this which have a specific base sequence at the 3' terminal side and a non-specific base sequence represented by Ambiguity Cord at the 5' terminal side as a primer.