Title:
METHOD FOR OBTAINING IMMORTALIZED ANTIBODY SECRETING CELL
Document Type and Number:
Japanese Patent JP2013255515
Kind Code:
A
Abstract:
To provide novel methods for immortalizing cells that secrete antibodies of one or more kinds of specific isotypes.
Polyclonal, oligoclonal, and monoclonal populations of cells obtained by using methods of this technology can be screened on the basis of the functional and/or binding activities of the antibodies which they secrete, for example directed to antigens of human or viral origin having medical interest, in cell culture conditions. Using these methods, human B cells that secrete antibodies binding to human Cytomegalovirus, Herpes Simplex Virus, or HSP60 protein have been efficiently immortalized with Epstein-Barr virus.
Inventors:
FUNARO ADA
GAROTTA GIANNI
MURPHY MARIANNE
GAROTTA GIANNI
MURPHY MARIANNE
Application Number:
JP2013181012A
Publication Date:
December 26, 2013
Filing Date:
September 02, 2013
Export Citation:
Assignee:
RIBOVAX BIOTECHNOLOGIES SA
International Classes:
C12N15/09; C07K16/00; C12N15/02; C12P21/08; C12Q1/68; C40B40/08; G01N33/53; G01N33/564
Domestic Patent References:
| JPH04211394A | 1992-08-03 | |||
| JPH11507640A | 1999-07-06 |
Foreign References:
| WO2004076677A2 | 2004-09-10 |
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Masaru Nakajima
Ryotaro Takei
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Masaru Nakajima
Ryotaro Takei