To provide a method for preparing a rat model, exhibiting carcinogenesis of primary liver cancer and its metastasis to the lung as a liver cancer model that matches human clinical examination.
In this method for preparing rat model of lung metastasis induced by a primary liver cancer, a nitroso compound is infused a multiple number of times in the abdominal cavity of a rat, at a rate of 20-200 mg/kg-body weight for eachdose; while a period of 4 weeks or longer that is free of nitroso compound infusion after the final infusion is set to obtain the rat model of lung metastasis induced by a primary liver cancer. The nitroso compound is, preferably, diethylnitrosamine, and the evaluation of anticancer agent and antimetastatic agent can be carried out by using the model rat.
IDA NOBUO
Masaki Hiroyuki