METHOD FOR PREPARING SAMPLE FOR DETECTION USABLE FOR DETECTING METHYLATION OF DNA, METHOD FOR DETECTING METHYLATION OF DNA, SAMPLE-TREATING LIQUID AND KIT

Title:

METHOD FOR PREPARING SAMPLE FOR DETECTION USABLE FOR DETECTING METHYLATION OF DNA, METHOD FOR DETECTING METHYLATION OF DNA, SAMPLE-TREATING LIQUID AND KIT

Document Type and Number:

Japanese Patent JP2007259750

Kind Code:

A

Abstract:

To provide a method for preparing a sample for detection by which an analyzing time can be shortened by simplifying a pretreatment method of a biosample usable for detecting the methylation of a DNA, and further to provide a method for detecting the methylation of the DNA, a sample-treating liquid and a kit.

The method for preparing the sample for the detection usable for detecting the methylation of the DNA includes a step for mixing a biosample containing a cell with the sample-treating liquid containing a surfactant for solubilizing the cell, and a step for adding a converting agent for converting an unmethylated cytosine contained in the DNA to other bases.

More Like This:

JP3572316	Methods for determining the presence or absence of cancer in a sample by determining the expression of peptides derived from the SSX gene, the SSX gene and the NY-ESO-1 gene, and their use.
JP2004532003	How to implement biochemical protocols in continuous flow in a microreactor
WO/1994/026922	METHODS AND COMPOSITIONS RELATING TO STEROL REGULATORY ELEMENT BINDING PROTEINS

Inventors:

KAJITA MASAHIRO
ISHIHARA HIDEMIKI

Application Number:

JP2006088553A

Publication Date:

October 11, 2007

Filing Date:

March 28, 2006

Export Citation:

Click for automatic bibliography generation Help

Assignee:

SYSMEX CORP

International Classes:

C12Q1/68; C12N15/09; G01N33/53; G01N33/566

Attorney, Agent or Firm:

Patent Business Corporation Suncrest International Patent Office

Previous Patent: METHOD FOR PROLIFERATING PLANT, CHARACTERIZED BY ETHYLENE TREATMENT

Next Patent: TRANSGENIC RICE PLANT EXPRESSING GLUTENIN SUBUNIT OF WHEAT