METHOD FOR PREPARING SPECIMEN FOR PCR METHOD

Title:

METHOD FOR PREPARING SPECIMEN FOR PCR METHOD

Document Type and Number:

Japanese Patent JPH04349900

Kind Code:

A

Abstract:

PURPOSE: To remove or reduce interferences caused by coexistent substances in a specimen by treating a preliminary liquid specimen before DNA is multiplied by a PCR method.

CONSTITUTION: A preliminary liquid specimen such as urine is treated with polyethlene glycol (preferably having a mol.wt. of 1000-50000) to prepare a liquid specimen for a PCR method. Preferably, an aqueous solution of polyethylene glycol is added to the preliminary liquid specimen and the mixture is allowed to stand at a low temperature for 6hrs to precipitate DNA and viruses, followed by suspending the precipitates in a buffer solution to provide a liquid specimen.

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Inventors:

HIRAI KANJI
HIRONAKA TAKASHI
YAMAGUCHI MASAKI
KITA HIROSHI

Application Number:

JP40625190A

Publication Date:

December 04, 1992

Filing Date:

December 07, 1990

Export Citation:

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Assignee:

IATRON LAB

International Classes:

C12Q1/68; C12N15/10; (IPC1-7): C12N15/10; C12Q1/68

Attorney, Agent or Firm:

Kenichi Morita

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